International
Tables for
Crystallography
Volume C
Mathematical, physical and chemical tables
Edited by E. Prince

© International Union of Crystallography 2006
International Tables for Crystallography (2006). Vol. C. ch. 3.1, p. 150

Section 3.1.1.6. Automated protein crystallization

P. F. Lindleya

a ESRF, Avenue des Martyrs, BP 220, F-38043 Grenoble CEDEX, France

3.1.1.6. Automated protein crystallization

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Several liquid-handling systems have been described that can automatically set up, reproducibly, a range of crystallization conditions (different protein concentrations, ionic strengths, amounts of organic precipitant, etc.) for the hanging-drop, sitting-drop, and microbatch methods. A useful introduction describing a system for mixing both buffered protein solutions and the corresponding reservoirs is given by Cox & Weber (1987[link]). Chayen, Shaw Stewart, Maeder & Blow (1990[link]) describe an automatic dispenser involving a bank of Hamilton syringes driven by stepper motors under computer control that can be used to set up small samples (2 µl or less) for microbatch crystallization (or hanging drops). Further systems have been described by Oldfield, Ceska & Brady (1991[link]), Eiselé (1993[link]), Soriano & Fontecilla-Camps (1993[link]), Sadaoui, Janin & Lewit-Bentley (1994[link]), and Chayen, Shaw Stewart & Baldock (1994[link]).

References

First citation Chayen, N. E., Shaw Stewart, P. D. & Baldock, P. (1994). New developments of the IMPAX small-volume automated crystallization system. Acta Cryst. D50, 456–458.Google Scholar
 First citation Chayen, N. E., Shaw Stewart, P. D., Maeder, D. L. & Blow, D. M. (1990). An automated system for micro-batch protein crystallization and screening. J. Appl. Cryst. 23, 297–302.Google Scholar
 First citation Cox, M. J. & Weber, P. C. (1987). Experiments with automated protein crystallization. J. Appl. Cryst. 20, 366–373.Google Scholar
 First citation Eiselé, J.-L. (1993). Preparation of protein crystallization buffers with a computer-controlled motorized pipette: PIPEX. J. Appl. Cryst. 26, 92–96.Google Scholar
 First citation Oldfield, T. J., Ceska, T. A. & Brady, R. L. (1991). A flexible approach to automated protein crystallization. J. Appl. Cryst. 24, 255–260.Google Scholar
 First citation Sadaoui, N., Janin, J. & Lewit-Bentley, A. (1994). TAOS: an automated system for protein crystallization. J. Appl. Cryst. 27, 622–626.Google Scholar
 First citation Soriano, T. M. B. & Fontecilla-Camps, J. C. (1993). ASTEC: an automated system for sitting-drop protein crystallization. J. Appl. Cryst. 26, 558–562.Google Scholar








































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