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International
Tables for Crystallography Volume F Crystallography of biological macromolecules Edited by M. G. Rossmann and E. Arnold © International Union of Crystallography 2006 |
International Tables for Crystallography (2006). Vol. F. ch. 19.4, p. 443
Section 19.4.6.5. Distance measurements and triangulation
aDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA, and bDepartments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA |
By measuring distances and radii of gyration, models of some large complexes have been created. These include a model of the relative positions of all 21 proteins in the small ribosomal subunit from E. coli (Capel et al., 1987![[link]](/graphics/greenarr.gif)
), a model of seven proteins from the large subunit (May et al., 1992) and a model of the DNA-dependant RNA polymerase (Stöckel et al., 1980b). Intramolecular conformational states of cholesterol esters have been observed using distance measurements on molecules chemically labelled in different regions (Burks & Engelman, 1981).
References
Burks, C. & Engelman, D. M. (1981). Cholesteryl myristate conformation in liquid crystalline mesophases determined by neutron scattering. Proc. Natl Acad. Sci. USA, 78, 6863–6867.Google Scholar
Capel, M. S., Engelman, D. M., Freeborn, B. R., Kjeldgaard, M., Langer, J. A., Ramakrishnan, V., Schindler, D. G., Schneider, D. K., Schoenborn, B. P. & Sillers, I. Y. (1987). A complete mapping of the proteins in the small ribosomal subunit of Escherichia coli. Science, 238, 1403–1406.Google Scholar
May, R. P., Nowotny, V., Nowotny, P., Voss, H. & Nierhaus, K. H. (1992). Inter-protein distances within the large subunit from Escherichia coli ribosomes. EMBO J. 11, 373–378.Google Scholar
Stöckel, P., May, R., Strell, I., Cejka, Z., Hoppe, W., Heumann, H., Zillig, W. & Crespi, H. L. (1980b). The subunit positions within RNA polymerase holoenzyme determined by triangulation of centre-to-centre distances. Eur. J. Biochem. 112, 419–423.Google Scholar
