International Tables for Crystallography (2006). Vol. F, ch. 19.6, pp. 451-463   | 1 | 2 |
doi: 10.1107/97809553602060000703

Chapter 19.6. Electron cryomicroscopy

Contents

  • 19.6. Electron cryomicroscopy  (pp. 451-463) | html | pdf | chapter contents |
    • 19.6.1. Abbreviations used  (p. 451) | html | pdf |
    • 19.6.2. The role of electron microscopy in macromolecular structure determination  (pp. 451-452) | html | pdf |
    • 19.6.3. Electron scattering and radiation damage  (pp. 452-453) | html | pdf |
      • 19.6.3.1. Elastic and inelastic scattering  (p. 452) | html | pdf |
      • 19.6.3.2. Radiation damage  (pp. 452-453) | html | pdf |
      • 19.6.3.3. Required properties of the illuminating electron beam  (p. 453) | html | pdf |
    • 19.6.4. Three-dimensional electron cryomicroscopy of macromolecules  (pp. 453-463) | html | pdf |
      • 19.6.4.1. Overview of conceptual steps  (pp. 453-454) | html | pdf |
      • 19.6.4.2. Classification of macromolecules  (pp. 454-455) | html | pdf |
      • 19.6.4.3. Specimen preparation  (pp. 455-456) | html | pdf |
      • 19.6.4.4. Microscopy  (pp. 456-458) | html | pdf |
      • 19.6.4.5. Selection and preprocessing of digitized images  (pp. 458-459) | html | pdf |
      • 19.6.4.6. Image processing and 3D reconstruction  (pp. 459-461) | html | pdf |
        • 19.6.4.6.1. 2D crystals  (pp. 459-460) | html | pdf |
        • 19.6.4.6.2. Helical particles  (pp. 460-461) | html | pdf |
        • 19.6.4.6.3. Icosahedral particles  (p. 461) | html | pdf |
      • 19.6.4.7. Visualization, modelling and interpretation of results  (pp. 461-462) | html | pdf |
      • 19.6.4.8. Solving the X-ray phase problem with cryo EM maps  (pp. 462-463) | html | pdf |
    • 19.6.5. Recent trends  (p. 463) | html | pdf |
    • References | html | pdf |
    • Figures
      • Fig. 19.6.2.1. Schematic diagram showing the principle of image formation and diffraction in the transmission electron microscope  (p. 451) | html | pdf |
      • Fig. 19.6.4.1. Flow diagram showing all the procedures involved in electron cryomicroscopy from sample preparation to map interpretation  (p. 453) | html | pdf |
      • Fig. 19.6.4.2. A display of the results at different stages of image processing of a digitized micrograph of a 2D crystal of bacteriorhodopsin  (p. 454) | html | pdf |
      • Fig. 19.6.4.3. Schematic diagram to illustrate the principle of 3D reconstruction  (p. 454) | html | pdf |
      • Fig. 19.6.4.4. Representative plots of the microscope contrast-transfer function (CTF) as a function of spatial frequency, for two different defocus settings (0.7 and 4.0 µm underfocus) and for a field-emission (light curve) or tungsten (dark curve) electron source  (p. 458) | html | pdf |
      • Fig. 19.6.5.1. Examples of macromolecules studied by cryo EM and 3D image reconstruction and the resulting 3D structures (bottom row) after cryo EM analysis  (p. 463) | html | pdf |
    • Tables
      • Table 19.6.4.1. Classification of macromolecules according to periodic order and symmetry  (p. 455) | html | pdf |
      • Table 19.6.4.2. Methods of three-dimensional image reconstruction  (p. 460) | html | pdf |