International Tables for Crystallography (2006). Vol. F. ch. 4.1, pp. 81-93   | 1 | 2 |
https://doi.org/10.1107/97809553602060000660

Chapter 4.1. General methods

Contents

  • 4.1. General methods  (pp. 81-93) | html | pdf | chapter contents |
    R. Giegé and A. McPherson
    • 4.1.1. Introduction  (p. 81) | html | pdf |
    • 4.1.2. Crystallization arrangements and methodologies  (pp. 81-86) | html | pdf |
      • 4.1.2.1. General considerations  (p. 81) | html | pdf |
      • 4.1.2.2. Batch crystallizations  (pp. 81-82) | html | pdf |
      • 4.1.2.3. Dialysis methods  (p. 82) | html | pdf |
      • 4.1.2.4. Vapour diffusion methods  (pp. 82-84) | html | pdf |
      • 4.1.2.5. Interface diffusion and the gel acupuncture method  (p. 84) | html | pdf |
      • 4.1.2.6. Crystallization in gelled media  (pp. 84-86) | html | pdf |
      • 4.1.2.7. Miscellaneous crystallization methods  (p. 86) | html | pdf |
      • 4.1.2.8. Seeding  (p. 86) | html | pdf |
    • 4.1.3. Parameters that affect crystallization of macromolecules  (pp. 86-88) | html | pdf |
      • 4.1.3.1. Crystallizing agents  (pp. 86-87) | html | pdf |
      • 4.1.3.2. Other chemical, physical and biochemical variables  (pp. 87-88) | html | pdf |
      • 4.1.3.3. Additives  (p. 88) | html | pdf |
    • 4.1.4. How to crystallize a new macromolecule  (pp. 88-89) | html | pdf |
      • 4.1.4.1. Rules and general principles  (p. 88) | html | pdf |
      • 4.1.4.2. Purity and homogeneity  (p. 88) | html | pdf |
      • 4.1.4.3. Sample preparation  (pp. 88-89) | html | pdf |
      • 4.1.4.4. Strategic concerns: a summary  (p. 89) | html | pdf |
    • 4.1.5. Techniques for physical characterization of crystallization  (pp. 89-91) | html | pdf |
      • 4.1.5.1. Techniques for studying prenucleation and nucleation  (pp. 89-90) | html | pdf |
      • 4.1.5.2. Techniques for studying growth mechanisms  (pp. 90-91) | html | pdf |
      • 4.1.5.3. Techniques for evaluating crystal perfection  (p. 91) | html | pdf |
    • 4.1.6. Use of microgravity  (pp. 91-93) | html | pdf |
      • 4.1.6.1. Why microgravity?  (p. 91) | html | pdf |
      • 4.1.6.2. Instrumentation  (p. 91) | html | pdf |
      • 4.1.6.3. Present results: a summary  (pp. 91-92) | html | pdf |
      • 4.1.6.4. Interpretation of data  (pp. 92-93) | html | pdf |
      • 4.1.6.5. The future of crystallization under microgravity  (p. 93) | html | pdf |
    • References | html | pdf |
    • Figures
      • Fig. 4.1.2.1. Principles of the major methods currently used to crystallize biological macromolecules  (p. 82) | html | pdf |
      • Fig. 4.1.2.2. Two versions of boxes for vapour diffusion crystallization  (p. 83) | html | pdf |
      • Fig. 4.1.2.3. Principle of the gel acupuncture method for the crystallization of proteins by counter-diffusion  (p. 84) | html | pdf |
      • Fig. 4.1.5.1. Visualization of the surface of yeast tRNAPhe crystals by AFM  (p. 90) | html | pdf |
    • Tables
      • Table 4.1.2.1. Factors affecting crystallization  (p. 83) | html | pdf |
      • Table 4.1.2.2. Crystallizing agents for protein crystallization  (pp. 85-86) | html | pdf |