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International
Tables for Crystallography Volume F Crystallography of biological macromolecules Edited by M. G. Rossmann and E. Arnold © International Union of Crystallography 2006 |
International Tables for Crystallography (2006). Vol. F. ch. 4.1, p. 84
Figure 4.1.2.3
a
Unité Propre de Recherche du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, F-67084 Strasbourg CEDEX, France, and bDepartment of Molecular Biology & Biochemistry, University of California at Irvine, Irvine, CA 92717, USA |
![[Figure 4.1.2.3]](/figures/Fafig4o1o2o3.gif)
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Figure 4.1.2.3
Principle of the gel acupuncture method for the crystallization of proteins by counter-diffusion. Capillaries containing the macromolecule solution are inserted into a gel, which is covered by a layer of crystallizing agent (CA); the setup is closed by a glass plate. The crystallizing-agent solution diffuses through the gel to the capillaries. The kinetics of crystal growth can be controlled by varying the CA concentration, the capillary volume (diameter and height) and its height in the gel. |