International Tables for Crystallography (2012). Vol. F, ch. 19.6, pp. 593-614   | 1 | 2 |
doi: 10.1107/97809553602060000872

Chapter 19.6. Electron cryomicroscopy of biological macromolecules

Contents

  • 19.6. Electron cryomicroscopy of biological macromolecules  (pp. 593-614) | html | pdf | chapter contents |
    • 19.6.1. Abbreviations used  (p. 593) | html | pdf |
    • 19.6.2. Introduction: macromolecular structure determination using electron microscopy  (p. 593) | html | pdf |
    • 19.6.3. Physics of electron scattering and radiation damage  (pp. 593-595) | html | pdf |
      • 19.6.3.1. Elastic and inelastic scattering  (p. 594) | html | pdf |
      • 19.6.3.2. Radiation damage  (pp. 594-595) | html | pdf |
      • 19.6.3.3. Required properties of the illuminating electron beam  (p. 595) | html | pdf |
    • 19.6.4. Three-dimensional electron cryomicroscopy of macromolecules  (pp. 595-601) | html | pdf |
      • 19.6.4.1. Overview of conceptual steps  (pp. 595-596) | html | pdf |
      • 19.6.4.2. Classification of macromolecules  (p. 597) | html | pdf |
      • 19.6.4.3. Specimen preparation  (pp. 597-599) | html | pdf |
      • 19.6.4.4. Microscopy  (pp. 599-600) | html | pdf |
      • 19.6.4.5. Selection and preprocessing of digitized images  (p. 601) | html | pdf |
    • 19.6.5. Image processing and 3D reconstruction  (pp. 601-604) | html | pdf |
      • 19.6.5.1. 2D crystals  (p. 603) | html | pdf |
      • 19.6.5.2. Helical particles  (p. 603) | html | pdf |
      • 19.6.5.3. Icosahedral particles  (pp. 603-604) | html | pdf |
      • 19.6.5.4. Electron cryo-tomography  (p. 604) | html | pdf |
    • 19.6.6. Visualization, modelling and interpretation of results  (pp. 604-605) | html | pdf |
    • 19.6.7. Trends  (pp. 605-606) | html | pdf |
    • References | html | pdf |
    • Figures
      • Fig. 19.6.3.1. Schematic diagram showing the principle of image formation and diffraction in the transmission electron microscope  (p. 594) | html | pdf |
      • Fig. 19.6.4.1. Flow diagram showing common procedures involved in cryoTEM from sample preparation to map interpretation  (p. 595) | html | pdf |
      • Fig. 19.6.4.2. A display of the results at different stages of image processing of a digitized micrograph of a 2D crystal of bacteriorhodopsin  (p. 596) | html | pdf |
      • Fig. 19.6.4.3. Schematic diagram to illustrate the principle of 3D reconstruction  (p. 597) | html | pdf |
      • Fig. 19.6.4.4. Representative plots of the microscope CTF as a function of spatial frequency, for two different defocus settings (0.7 and 4.0 µm underfocus) and for a field-emission (light curve) or tungsten (dark curve) electron source  (p. 600) | html | pdf |
      • Fig. 19.6.7.1. Examples of macromolecules studied by cryoTEM and 3D image reconstruction and the resulting 3D structures (bottom row) after cryoTEM analysis  (p. 605) | html | pdf |
    • Tables
      • Table 19.6.4.1. Classification of macromolecules according to periodic order and symmetry  (p. 598) | html | pdf |
      • Table 19.6.5.1. Methods of three-dimensional image reconstruction  (p. 602) | html | pdf |