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 Results for DC.creator="A." AND DC.creator="McPherson"
General methods
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, ch. 4.1, pp. 99-121 [ doi:10.1107/97809553602060000812 ]
... 4.1. General methods The preparation of well diffracting crystals is a crucial step of any crystallographic project. This chapter defines the ... viewpoint, and ways to enhance crystal perfection are given. In a practical perspective, strategies to efficiently prepare crystals for structural biology ... ranging from as little as 25 up to 90%. A direct consequence is that, although morphologically indistinguishable, crystals of ...

Strategic concerns: a summary
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.6.4, p. 115 [ doi:10.1107/97809553602060000812 ]
Strategic concerns: a summary 4.1.6.4. Strategic concerns: a summary Homogeneity and stability. Perhaps the most important property of a system to be crystallized is its purity. Crystallization presupposes ...
     [more results from section 4.1.6 in volume F]

Crystal perfection
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.5.4, pp. 112-113 [ doi:10.1107/97809553602060000812 ]
... to analyse crystals of high perfection, in other words, with a minimum of defects, disorder, impurity incorporation and internal stress. Such ... are responsible for increases in the diffuse background scatter and a broadening of diffraction intensities. These defects are difficult to monitor ... diffraction topography. Both techniques have been refined with lysozyme as a test case and were used for comparative analysis of ...
     [more results from section 4.1.5 in volume F]

Seeding
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.4.4, pp. 110-111 [ doi:10.1107/97809553602060000812 ]
... of nuclei is limiting, or spontaneous nucleation occurs at such a profound level of supersaturation that poor growth results. In such cases, it is desirable to induce growth in a directed fashion at low levels of supersaturation. This can be accomplished by seeding a metastable, supersaturated protein solution with crystals from earlier trials. ...
     [more results from section 4.1.4 in volume F]

Miniaturization, automation and robotics
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.3.6, p. 107 [ doi:10.1107/97809553602060000812 ]
... in most cases, nanolitres in some. This, in turn, produces a requirement for automated, microphotographic visualization instruments, and complex storage and ... handling systems, and their associated expense. On the other hand, a great advantage emerges in that they can perform enormous numbers ... little biological sample. This, in turn, relieves the investigator of a significant burden in terms of preparing and purifying macromolecules. ...
     [more results from section 4.1.3 in volume F]

Purity and homogeneity
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.2.4, pp. 103-104 [ doi:10.1107/97809553602060000812 ]
... alter macromolecular solubility, or interfere with nucleation and crystal growth (McPherson et al., 1996, 2004; Moreno et al., 2005; Thomas et ... causes are partial hydrolysis and post-synthetic modifications. Proteolysis represents a major difficulty that must be overcome during protein isolation. In ... such subtle degradations or modifications explain why altered versions of a macromolecule can be the worst contaminants for its crystallization. ...
     [more results from section 4.1.2 in volume F]

Crystallization principles
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.1.2, pp. 99-100 [ doi:10.1107/97809553602060000812 ]
... principles as that of small molecules (Giegé et al., 1995; McPherson et al., 1995; Rosenberger, 1996; Chernov, 2003; McPherson & Giegé, 2007). It is, similarly, a multiparametric process. The difference compared with conventional crystal growth ...
     [more results from section 4.1.1 in volume F]

The future of protein crystal growth
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.7, p. 115 [ doi:10.1107/97809553602060000812 ]
... quality X-ray diffraction data can frequently be obtained from a single crystal of dimensions in the range of 20 to ... many crystals in the millimetre size range were required for a structure analysis. A consequence of this is that attention is turning increasingly ...

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