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The preparation of heavy-atom derivatives of protein crystals for use in multiple isomorphous replacement and anomalous scattering
Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L.  International Tables for Crystallography (2012). Vol. F, ch. 12.1, pp. 317-326 [ doi:10.1107/97809553602060000837 ]
... solvent (e.g. acetonitrile). Tetrakis(acetoxymercurio)methane (TAMM) and di-m-iodobis(ethylenediamine)diplatinum(II) nitrate (PIP) have better solubility in ... incorporation has also been reported in non-auxotrophic bacterial strains, E. coli strain XA90 (Labahn et al., 1996), in a mammalian ... ICRF and the Wellcome Trust for support. References Abraham, D. J., Phillips, S. E. V. & Kennedy, P. E. (1983). ...

Analogues of amino acids
Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L.  International Tables for Crystallography (2012). Vol. F, Section 12.1.7, pp. 324-325 [ doi:10.1107/97809553602060000837 ]
... incorporation has also been reported in non-auxotrophic bacterial strains, E. coli strain XA90 (Labahn et al., 1996), in a mammalian ... promising new approach for X-ray structure analysis of proteins. J. Mol. Biol. 271, 1-8. Ceska, T. A., Sayers, J. R., Stier, G. & Suck, D. (1996). A helical ...

Metal-ion replacement in metalloproteins
Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L.  International Tables for Crystallography (2012). Vol. F, Section 12.1.6, p. 324 [ doi:10.1107/97809553602060000837 ]
Metal-ion replacement in metalloproteins 12.1.6. Metal-ion replacement in metalloproteins The metal-ion cofactor can sometimes be displaced by dialysis or diffusion by a heavy-atom solution, but usually the cofactor is first removed by a chelating agent (e.g. EDTA) or by acidification. These are best carried out on the ...

Polynuclear reagents
Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L.  International Tables for Crystallography (2012). Vol. F, Section 12.1.5.7, p. 324 [ doi:10.1107/97809553602060000837 ]
... solvent (e.g. acetonitrile). Tetrakis(acetoxymercurio)methane (TAMM) and di-m-iodobis(ethylenediamine)diplatinum(II) nitrate (PIP) have better solubility in ... al. (1996). References O'Halloran, T. V., Lippard, S. J., Richmond, T. J. & Klug, A. (1987). Multiple heavy-atom reagents for ...
     [more results from section 12.1.5 in volume F]

Amino acids as ligands
Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L.  International Tables for Crystallography (2012). Vol. F, Section 12.1.4, pp. 320-321 [ doi:10.1107/97809553602060000837 ]
... useful derivatives. References Ely, K. R., Girling, R. L., Schiffer, M., Cunningham, D. E. & Edmundson, A. B. (1973). Preparation and properties of crystals ... 12, 4233-4237. Lipscomb, W. N., Reeke, G. N., Hartsuck, J. A., Quiocho, F. A. & Bethge, P. H. (1970). ...

Effect of concentration, time of soak and temperature on heavy-atom binding
Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L.  International Tables for Crystallography (2012). Vol. F, Section 12.1.3.7, p. 320 [ doi:10.1107/97809553602060000837 ]
Effect of concentration, time of soak and temperature on heavy-atom binding 12.1.3.7. Effect of concentration, time of soak and temperature on heavy-atom binding Most heavy-atom derivatives are prepared by diffusing or dialysing the compound into the crystals. Concentrations have typically ranged from 0.1-100.0mM. Occasionally, concentrations as low ...
     [more results from section 12.1.3 in volume F]

Heavy-atom data bank
Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L.  International Tables for Crystallography (2012). Vol. F, Section 12.1.2, pp. 317-318 [ doi:10.1107/97809553602060000837 ]
Heavy-atom data bank 12.1.2. Heavy-atom data bank The heavy-atom data bank (HAD) is a computer-based archival file system that contains experimental and derived information from successful multiple isomorphous replacement analyses in the determination of protein crystal structures. HAD is available at http://www.sbg.bio.ic.ac.uk/had/ . The data ...

Introduction
Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L.  International Tables for Crystallography (2012). Vol. F, Section 12.1.1, p. 317 [ doi:10.1107/97809553602060000837 ]
... MIRAS. References Bernstein, F. C., Koetzle, T. F., Williams, G. J. B., Meyer, E. F. Jr, Brice, M. D., Rodgers, J. R., Kennard, O., Shimanouchi, T. & Tasumi, ...

Use of the heavy-atom data bank to select derivatives
Carvin, D., Islam, S. A., Sternberg, M. J. E. and Blundell, T. L.  International Tables for Crystallography (2012). Vol. F, Section 12.1.8, p. 325 [ doi:10.1107/97809553602060000837 ]
Use of the heavy-atom data bank to select derivatives 12.1.8. Use of the heavy-atom data bank to select derivatives The heavy-atom data bank is probably best exploited by first investigating the most commonly used heavy-atom reagents with a view to obtaining mercury, platinum and uranyl derivatives that ...

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