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International
Tables for Crystallography Volume F Crystallography of biological macromolecules Edited by M. G. Rossmann and E. Arnold © International Union of Crystallography 2006 |
International Tables for Crystallography (2006). Vol. F. ch. 19.4, p. 443
Section 19.4.6.4. Specific deuteration, combination with X-ray measurements
aDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA, and bDepartments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA |
Many recent measurements use specific labelling and combine information with data from small-angle X-ray scattering. Studies of ligand binding (Bilgin et al., 1998![[link]](/graphics/greenarr.gif)
), hydration layers (Svergun et al., 1998), troponin complexes (Olah et al., 1994) and ribosomes (Svergun et al., 1996) are examples that illustrate the approaches.
References
Bilgin, N., Ehrenberg, M., Ebel, C., Zaccai, G., Sayers, Z., Koch, M. H. J., Svergun, D. I., Barberato, C., Volkov, V., Nissen, P. & Nyborg, J. (1998). Solution structure of the ternary complex between aminoacyl-tRNA, elongation factor Tu, and guanosine triphosphate. Biochemistry, 37, 8163–8172.Google Scholar
Olah, G. A., Rokop, S. E., Wang, C. L., Blechner, S. L. & Trewhella, J. (1994). Troponin I encompasses an extended troponin C in the Ca(2+)-bound complex: a small-angle X-ray and neutron scattering study. Biochemistry, 33, 8233–8239.Google Scholar
Svergun, D. I., Koch, M. H., Pedersen, J. S. & Serdyuk, I. N. (1996). Structural model of the 50S subunit of E. coli ribosomes from solution scattering. Basic Life Sci. 64, 149–174.Google Scholar
Svergun, D. I., Richard, S., Koch, M. H., Sayers, Z., Kuprin, S. & Zaccai, G. (1998). Protein hydration in solution: experimental observation by X-ray and neutron scattering. Proc. Natl Acad. Sci. USA, 95, 2267–2272.Google Scholar
