International
Tables for Crystallography Volume F Crystallography of biological macromolecules Edited by M. G. Rossmann and E. Arnold © International Union of Crystallography 2006 |
International Tables for Crystallography (2006). Vol. F. ch. 23.2, pp. 585-587
|
Novel features of molecular recognition and electrostatic interactions of these two tetrahedral oxyanions have emerged from our crystallographic and functional studies of the phosphate-binding protein (PBP) and sulfate-binding protein (SBP), which serve as extremely specific initial receptors for ATP-binding cassette (ABC)-type active transport or permease in bacterial cells. The complexes of these proteins have Kd values in the low µM range. Although phosphate and sulfate are structurally similar, at physiological pH PBP and SBP exhibit no overlap in specificity (Medveczky & Rosenberg, 1971; Pardee, 1966
; Jacobson & Quiocho, 1988
). This stringent specificity prevents one tetrahedral oxyanion nutrient from becoming an inhibitor of transport for the other. The specificity of the PBP-dependent phosphate transport system is also shared by other phosphate transport systems in eukaryotic cells and across brush borders and into mitochondria.
As described below, discrimination between anions is based solely on the protonation state of the ligand. Sulfate, a conjugate base of a strong acid, is completely ionized at pH values above 3, whereas phosphate, a conjugate base of a weak acid, remains protonated up to pH 13.
The structure of the PBP–phosphate complex was initially determined at 1.7 Å resolution (Luecke & Quiocho, 1990). The resolution has been pushed to an ultra high resolution of 0.98 Å, the first reported for a protein with a molecular weight as high as 34 kDa with a bound ligand (Wang et al., 1997
). The bound phosphate is completely desolvated and sequestered in the protein cleft between two domains. It makes 12 hydrogen bonds with the proteins (11 with donor groups and one with an acceptor group), as well as one salt link to an Arg that is in turn salt-linked to an Asp residue (Fig. 23.2.5.1)
. The distances of the 12 hydrogen bonds between phosphate and PBP obtained from the ultra high resolution structure range from 2.432 to 2.906 Å (Wang et al., 1997
). The Asp56 carboxylate, the lone acceptor group, plays two key roles in conferring the exquisite specificity of PBP. It recognizes, by way of the hydrogen bond, a proton on the phosphate and presumably disallows, by charge repulsion, the binding of a fully ionized sulfate dianion (Luecke & Quiocho, 1990
).
The SBP binding-site cleft is also tailor-made for sulfate (Pflugrath & Quiocho, 1985). In keeping with the stringent specificity of SBP for fully ionized tetrahedral oxyanions (Pardee, 1966
; Jacobson & Quiocho, 1988
), the bound sulfate, which is also completely dehydrated and buried, is held in place by seven hydrogen bonds made entirely with donor groups from uncharged polar residues of the protein (Fig. 23.2.5.2)
(Pflugrath & Quiocho, 1985
). The absence of a hydrogen-bond acceptor group accounts for the inability of SBP to bind phosphate. Interestingly, the absence of a salt link and the formation of five fewer hydrogen bonds with the bound sulfate (Fig. 23.2.5.2b)
than with the bound phosphate (Fig. 23.2.5.1b)
do not make the affinity of the SBP–sulfate complex any weaker than that of the PBP–phosphate complex. In fact, the sulfate binds 10–20 times more tightly to SBP (Pardee, 1966
; Jacobson & Quiocho, 1988
). Also, the hydration energies of both anions are likely to be similar.
The ability of PBP and SBP to differentiate each oxyanion ligand through the presence or absence of proton(s) is an extremely high level of sophistication in molecular recognition. The importance of complete hydrogen bonding in recognition of buried ligands is powerfully demonstrated in PBP and SBP. As the sulfate is fully ionized (i.e. possesses no hydrogen at physiological pH), repulsion occurs at Asp56 of PBP specifically for this dianion. On the other hand, SBP is unable to bind phosphate because it contains no hydrogen-bond acceptor in the binding site. Significantly, despite the potential for a large number of matched hydrogen-bonding pairs, a single mismatched hydrogen bond (e.g. a fully ionized sulfate providing no proton for interaction with Asp56 of PBP and no acceptor group in SBP for a phosphate proton) represents a binding energy barrier of 6–7 kcal mol−1 (1 kcal mol−1 = 4.184 kJ mol−1).
A novel finding of further paramount importance and wide implication is how the isolated charges of the protein-bound phosphate and sulfate are stabilized. No counter-charged residues or cations are associated with the sulfate completely buried in SBP. Although a salt link involving Arg135 is formed with the phosphate bound to PBP, it is shared with an Asp residue (Fig. 23.2.5.1b). Moreover, site-directed mutagenesis studies indicate that phosphate binding is quite insensitive to modulation of the salt link (Yao et al., 1996
). These findings are a powerful demonstration of how a protein is able to stabilize the charges by means other than salt links. Experimental and computational studies indicate that local dipoles, including the hydrogen-bonding groups and the backbone NH groups from the first turn of helices, immediately surrounding the sulfate and phosphate are responsible for charge stabilization (Pflugrath & Quiocho, 1985
; Quiocho et al., 1987
; Åqvist et al., 1991
; He & Quiocho, 1993
; Yao et al., 1996
; Ledvina et al., 1996
). Helix macrodipoles play little or no role in charge stabilization of the anions. The same principle of charge stabilization by local dipoles also applies for the following buried uncompensated ionic groups: Arg151 of the arabinose-binding protein (Quiocho et al., 1987
), the zwitterionic leucine ligand bound to the leucine/isoleucine/valine-binding protein (Quiocho et al., 1987
), the potassium in the pore of the potassium channel (Doyle et al., 1998
) and Arg56 of synaptobrevin-II in a SNARE complex (Sutton et al., 1998
).
The ultra high resolution refined structure of the PBP–phosphate complex is the first to show structurally the formation of an extremely short hydrogen bond (2.432 Å) between the Asp56 carboxylate of PBP and phosphate. Although this short hydrogen bond is within the proposed range of low-barrier hydrogen bonds with estimated energies of 12–24 kcal mol−1 (Hibbert & Emsley, 1990), its contribution to phosphate binding affinity has been assessed to be no better than that of a normal hydrogen bond (Wang et al., 1997
). Thus, a unique role for short hydrogen bonds in biological systems, such as in enzyme catalysis (Gerlt & Gassman, 1993
; Cleland & Kreevoy, 1994
), remains controversial.
23.2.5.3. Non-complementary negative electrostatic surface potential of protein sites specific for anions
The presence of an uncompensated negatively charged Asp56 is unusual for an anion-binding site, as observed in PBP. In fact, a related discovery of profound ramification is that the binding-cleft region of PBP has an intense negative electrostatic surface potential (Fig. 23.2.5.3a) (Ledvina et al., 1996
). Non-complementarity between the surface potential of a binding region and an anion ligand is not unique to PBP. We have reported similar findings for SBP, a DNA-binding protein, and, even more dramatically, for the redox protein flavodoxin (Fig. 23.2.5.3b)
(Ledvina et al., 1996
). Evidently, for proteins such as these, which rely on hydrogen-bonding interactions with only uncharged polar residues for anion binding and electrostatic balance, a non-complementary surface potential is not a barrier to binding. This conclusion is supported by very recent fast kinetic studies of binding of phosphate to PBP and the effect of ionic strength on binding (Ledvina et al., 1998
).
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