Table 24.4.6.1

Gilliland, G. L.; Tung, M.; Ladner, J. E.

doi:10.1107/97809553602060000721

International
Tables for
Crystallography
Volume F
Crystallography of biological macromolecules
Edited by M. G. Rossmann and E. Arnold

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International Tables for Crystallography (2006). Vol. F. ch. 24.4, pp. 672-673

Table 24.4.6.1

G. L. Gilliland,^a ^* M. Tung^a and J. E. Ladner^a

^aCenter for Advanced Research in Biotechnology of the Maryland Biotechnology Institute and National Institute of Standards and Technology, 9600 Gudelsky Dr., Rockville, MD 20850, USA
Correspondence e-mail: gary.gilliland@nist.gov

Table 24.4.6.1 | top | pdf |
Crystallization conditions for endonucleases

Endonuclease	Crystal data (space group, unit cell)	Crystallization method	Protein concentration (mg ml⁻¹)	Chemical additives to reservoir	pH	T (°C)	Reference(s)
BamHI	C2 76.4, 46.0, 69.4 Å, 110.5°	Vapour diffusion with microseeding	12.5	10% Glycerol, 0.02 M potassium phosphate, 12% polyethylene glycol 8K	6.9	20	Newman et al. (1994)
BamHI:12bp DNA	$[P2_{1}2_{1}2_{1}]$ 108.8, 81.9, 68.8 Å	Vapour diffusion	10.8	5% Glycerol, 12% polyethylene glycol 8K, 0.15 M potassium chloride	6.9–7.6	22–24	Strzelecka et al. (1994)
Crf10I, type II restriction	I222 64.5, 81.3, 119.7 Å	Vapour diffusion	6.0	1.0 M Ammonium acetate, 0.075 M MES	6.5–7.5	20	Bozic et al. (1996)
EcoRV, type II restriction	$[P2_{1}2_{1}2_{1}]$ 58.2, 71.7, 136.0 Å	Vapour diffusion	3.3–5.3	0.18 M Sodium chloride, 10% polyethylene glycol 4K	7.0–7.8	20–24	D'Arcy et al. (1985); Winkler et al. (1993)
EcoRV, type II restriction:11-mer DNA	P1 49.4, 50.2, 64.0 Å, 96.5, 109.1, 108.1°	Batch	6.4–12.9	0.0043 M Phosphate buffer, 0.1073 M sodium chloride, 0.00040 M EDTA, 0.00040 M dithiothreitol, 0.0043 M cacodylate, 0.8–1.4% polyethylene glycol 4K	6.0–7.5	22–24	Kostrewa & Winkler (1995)
EcoRV, type II restriction:cognate DNA	$[C222_{1}]$ 60.2, 78.4, 371.3 Å	Vapour diffusion	n/a	0.1 M Sodium phosphate, 0.08 M sodium chloride	6.4–6.9	19–22	Winkler et al. (1991, 1993)
EcoRV, type II restriction:non-cognate DNA	$[P2_{1}]$ 68.4, 79.6, 6.4 Å, 104.6°	Vapour diffusion	7.0–10.0	0.1 M Sodium chloride, 0.02 M MES	6.4–6.9	19–22	Winkler et al. (1991, 1993)
EcoRV, type II restriction:product DNA	P1 49.3, 50.3, 63.9 Å, 96.7, 108.8, 108.4°	Microbatch	6.4	0.0043 M Phosphate buffer, 0.1073 M sodium chloride, 0.0004 M EDTA, 0.0004 M dithiothreitol, 0.0043 M cacodylate, 1–2% polyethylene glycol 4K, 0.0043 M magnesium chloride	6.0–7.5	4–22	Kostrewa & Winkler (1995)
II, DNA repair [4Fe–4S]	$[P2_{1}2_{1}2_{1}]$ 48.5, 65.8, 86.8 Å	Dialysis and macroseeding	n/a	5.0% Glycerol, 0.0003 M sodium azide, 0.1 M sodium chloride, 0.005 M HEPES	7.0	15	Kuo, McRee, Cunningham & Tainer (1992); Kuo, McRee, Fisher et al. (1992)
PvuII	$[P2_{1}2_{1}2]$ 84.2, 106.2, 46.9 Å	Vapour diffusion	2.5	20–50% Saturated ammonium sulfate	5.0	18	Athanasiadis & Kokkinidis (1991)
PuvII:cognate DNA	$[P2_{1}2_{1}2_{1}]$ 95.8, 86.3, 48.5 Å	Vapour diffusion	9.6	0.0001 M EDTA, 2.5–3.6% polyethylene glycol 4K, 0.0155–0.0225 M sodium acetate	4.5	16	Balendiran et al. (1994)
RuvC specific for Holliday junctions	$[P2_{1}]$ 72.8, 139.6, 32.4 Å, 93.0°	Microdialysis	8.0	0.05 M TrisHCl, 7.5% glycerol, 0.001 M EDTA, 0.001 M dithiothreitol, 0.3–0.4 M sodium cloride	8.0	22–24	Ariyoshi et al. (1994)
V, mutant E23Q	$[P2_{1}]$ 41.4, 40.1, 37.4 Å, 90.4°	Vapour diffusion	10.0	0.05 M Potassium chloride, 0.008 M sodium cacodylate, 15% polyethylene glycol 400	4.5–8.0	4	Morikawa et al. (1995)
V, mutant R3Q	$[P2_{1}]$ 41.4, 40.7, 37.4 Å, 90.1°	Vapour diffusion	10.0	0.05 M Potassium chloride, 0.008 M sodium cacodylate, 15% polyethylene glycol 400	4.5–8.0	4	Morikawa et al. (1995)
V	$[P2_{1}]$ 41.4, 40.1, 37.6 Å, 90.01°	Vapour diffusion	10.0	0.05 M Potassium chloride, 0.008 M sodium cacodylate, 15% polyethylene glycol 400	4.5	4	Morikawa et al. (1988, 1992, 1995)
V, mutant E23D	$[P2_{1}]$ 41.7, 40.2, 37.1 Å, 92°	Vapour diffusion	10.0	0.05 M Potassium chloride, 0.008 M sodium cacodylate, 15% polyethylene glycol 400	4.5–8.0	4	Morikawa et al. (1995)
Sm₁	$[P2_{1}2_{1}2_{1}]$ 69.0, 106.7, 74.8 Å	Vapour diffusion	10.0	0.01 M TrisHCl, 1.2–1.6 M ammonium sulfate	8.3	4	Bannikova et al. (1991)
Extracellular	$[P2_{1}2_{1}2_{1}]$ 106.7, 74.5, 68.9 Å	Dialysis	8.0	1.0–1.7 M Ammonium sulfate, 0.05 M sodium phosphate	6.0	4	Miller et al. (1991)
Restriction, FokI:20pb DNA	$[P2_{1}]$ 65.6, 119.3, 71.5 Å, 101.4°	Vapour diffusion with macroseeding	10.0	1.1 M Ammonium sulfate, 0.5 M MES, 0.2 M potassium chloride, 0.0005 M dithiothreitol, 0.0005 M EDTA, 5% glycerol	6.0	20	Hirsch et al. (1997); Wah et al. (1997)