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International
Tables for Crystallography Volume F Crystallography of biological macromolecules Edited by M. G. Rossmann and E. Arnold © International Union of Crystallography 2006 |
International Tables for Crystallography (2006). Vol. F. ch. 25.2, p. 721
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The hybrid models described above are used as the main tool for obtaining as full a protein model as possible from the map calculated with the initial phases.
Given the information contained in the hybrid model in the traditional form of stereochemical restraints, reciprocal-space refinement can work more efficiently, new improved phases can be obtained and a more accurate and complete protein model can be constructed. The new hybrid model can be re-input to refinement and these steps can be iterated so that improved phases result in construction of ever larger parts of the protein. An almost complete protein model can be obtained in a fully automated way.
