International
Tables for
Crystallography
Volume F
Crystallography of biological macromolecules
Edited by M. G. Rossmann and E. Arnold

© International Union of Crystallography 2006
International Tables for Crystallography (2006). Vol. F. ch. 3.1, p. 67   | 1 | 2 |

Figure 3.1.3.1 

S. H. Hughesa and A. M. Stockb*

a National Cancer Institute, Frederick Cancer R&D Center, Frederick, MD 21702-1201, USA, and bCenter for Advanced Biotechnology and Medicine, Howard Hughes Medical Institute and University of Medicine and Dentistry of New Jersey – Robert Wood Johnson Medical School, 679 Hoes Lane, Piscataway, NJ 08854–5627, USA
Correspondence e-mail:  stock@cabm.rutgers.edu

[Figure 3.1.3.1]
Figure 3.1.3.1

Creating an expression construct. PCR can be used to amplify the coding region of interest, providing that a suitable template is available. PCR primers should be designed to contain one or more restriction sites that can be conveniently used to subclone the fragment into the desired expression vector. It is often possible to choose vectors and primers such that a single PCR product can be ligated to multiple vectors. The ability to test several expression systems simultaneously is advantageous, since it is impossible to predict which vector/host system will give the most successful expression of a specific protein.