International Tables for Crystallography

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International Tables for Crystallography (2006). Vol. F, Section 3.1.5.3
Preparing recombinant proteins for X-ray crystallography
S. H. Hughes and A. M. Stock. International Tables for Crystallography (2012). Vol. F, ch. 3.1, pp. 75-91  [ doi:10.1107/97809553602060000810 ]

Abstract

It is possible to use recombinant DNA techniques to produce most proteins in quantities sufficient for crystallography. This chapter discusses issues (and problems) relevant to choosing methods appropriate for preparing recombinant proteins for X-ray crystallography. The aim is to help readers understand both the extant problems and the available solutions, so that, armed with a general understanding of the issues, they can more easily confront a variety of specific projects. Specific topics covered include protein engineering an expression construct, expression systems, protein purification and the characterization of the purified product.


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About International Tables for Crystallography

International Tables for Crystallography is the definitive resource and reference work for crystallography. The multi-volume series comprises articles and tables of data relevant to crystallographic research and to applications of crystallographic methods in all sciences concerned with the structure and properties of materials.