The electron-density map at 2 Å resolution
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.10,
p.
[ doi:10.1107/97809553602060000899 ]
of significant features of lower density. All the members of the lysozyme group participated in drawing the maps.
To illustrate the result that was obtained, sections z = 35/60 to 44/60 of the electron-density map are shown in Fig.
25.1.3.9 ...
Map interpretation and model building
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.11,
p.
[ doi:10.1107/97809553602060000899 ]
Glu 35, Asp 52, Asp 101 and Asp103, are shown by red hemispheres (Blake et al., 1967).
References
Blake, C. C. F., Johnson, L. N., Mair, G. A., North, A. C. T.,
Phillips,
D.
C. & Sarma, V. R. (1967 ...
Analysis of the structure at 2 Å resolution
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3,
p.
[ doi:10.1107/97809553602060000899 ]
North, A. C. T. &
Phillips,
D.
C. (1964). Adaptation of a linear diffractometer to measure three X-ray reflections quasi-simultaneously. J. Sci. Instrum. 41, 421–425. Google Scholar
Blake, C. C. F. (1968). The preparation ...
Final refinement of heavy-atom parameters
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.8,
p.
[ doi:10.1107/97809553602060000899 ]
these scattering curves were fitted to curves of the type . With this modification, Pangloss was used with the type II crystal centric data to obtain the heavy-atom parameters shown in Table
25.1.3.2 .
Table
25.1.3.2 | top | pdf ...
Intensity measurements
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.2,
p.
[ doi:10.1107/97809553602060000899 ]
and, after processing by the methods described below, they were used in September 1964 to calculate a new image of the structure at 6 Å resolution.
References
Arndt, U. W., North, A. C. T. &
Phillips,
D.
C. (1964). Adaptation ...
Experimental methods
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.4.1,
p.
[ doi:10.1107/97809553602060000899 ]
the crystal-mounting axis (φ). These measurements were used during data processing in an improved method of absorption correction devised by North et al. (1968) .
References
North, A. C. T.,
Phillips,
D.
C. & Mathews, F ...
Heavy-atom derivatives at 2 Å resolution
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.1,
p.
[ doi:10.1107/97809553602060000899 ]
...
The second low-resolution map at 6 Å
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.3,
p.
[ doi:10.1107/97809553602060000899 ]
of stronger rod-like features suggestive of α-helices. Two of these were prominent, running upwards from right to left, in the view of the new model shown in Fig.
25.1.3.3 .
Figure
25.1.3.3
| top | pdf |
Solid model ...
Data processing
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.5,
p.
[ doi:10.1107/97809553602060000899 ]
to that of the other. Where the plots were not linear, so that a simple form of correction was not applicable, the entire set of measurements was usually rejected.
References
North, A. C. T.,
Phillips,
D.
C. & Mathews, F. S. (1968 ...
Diffractometer output
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.4.2,
p.
[ doi:10.1107/97809553602060000899 ]
punched paper tape for immediate transfer to the computer. An example of this record is shown in Fig.
25.1.3.4 .
Figure
25.1.3.4
| top | pdf |
Paper-tape output from the triple-counter linear diffractometer, showing ...
