The electron-density map at 2 Å resolution
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.10,
p.
[ doi:10.1107/97809553602060000899 ]
of significant features of lower density. All the members of the lysozyme group participated in drawing the maps.
To illustrate the result that was obtained, sections z = 35/60 to 44/60 of the electron-density map are shown in Fig.
25.1.3.9 ...
Map interpretation and model building
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.11,
p.
[ doi:10.1107/97809553602060000899 ]
that the Canfield sequence (Fig.
25.1.3.10) was to be preferred in every respect.
A stereo-photograph of this model (Blake et al., 1967) is shown in Fig.
25.1.3.12 in a view that shows the most striking feature of the molecule. The molecular face ...
Analysis of the structure at 2 Å resolution
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3,
p.
[ doi:10.1107/97809553602060000899 ]
with constant h and − h . The ratios were then plotted against h, as shown in Fig.
25.1.3.6 .
Figure
25.1.3.5
| top | pdf |
Asymmetric mounting of a protein crystal with its mother liquor in a capillary tube ...
Final refinement of heavy-atom parameters
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.8,
p.
[ doi:10.1107/97809553602060000899 ]
these scattering curves were fitted to curves of the type . With this modification, Pangloss was used with the type II crystal centric data to obtain the heavy-atom parameters shown in Table
25.1.3.2 .
Table
25.1.3.2 | top | pdf ...
Intensity measurements
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.2,
p.
[ doi:10.1107/97809553602060000899 ]
the crystal and counter settings for reflections in levels adjacent to the flat-cone level are identical to one another and closely similar to those for reflections in the flat cone. This setting is illustrated in Fig.
25.1.3.1 ...
Experimental methods
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.4.1,
p.
[ doi:10.1107/97809553602060000899 ]
...
Heavy-atom derivatives at 2 Å resolution
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.1,
p.
[ doi:10.1107/97809553602060000899 ]
...
The second low-resolution map at 6 Å
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.3,
p.
[ doi:10.1107/97809553602060000899 ]
of stronger rod-like features suggestive of α-helices. Two of these were prominent, running upwards from right to left, in the view of the new model shown in Fig.
25.1.3.3 .
Figure
25.1.3.3
| top | pdf |
Solid model ...
Data processing
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.5,
p.
[ doi:10.1107/97809553602060000899 ]
25.1.3.6 .
Figure
25.1.3.5
| top | pdf |
Asymmetric mounting of a protein crystal with its mother liquor in a capillary tube. Anomalous-scattering differences would be seriously affected in (b ...
Diffractometer output
Blake, C. C. F.,
Fenn, R. H.,
Johnson, L. N.,
Koenig, D. F.,
Mair, G. A.,
North, A. C. T.,
Oldham, J. W. H.,
Phillips, D. C.,
Poljak, R. J.,
Sarma, V. R. and
Vernon, C. A.,
International Tables for Crystallography
(2012).
Vol. F,
Section 25.1.3.4.2,
p.
[ doi:10.1107/97809553602060000899 ]
punched paper tape for immediate transfer to the computer. An example of this record is shown in Fig.
25.1.3.4 .
Figure
25.1.3.4
| top | pdf |
Paper-tape output from the triple-counter linear diffractometer, showing ...
