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 Results for DC.creator="A." AND DC.creator="M." AND DC.creator="Stock" in section 3.1.3 of volume F
Addition of tags or domains
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.3.3, p. 77 [ doi:10.1107/97809553602060000810 ]
... or domains In some cases it is useful to add a small peptide tag or a larger protein to either the amino or carboxyl terminus of ... tags, such as the maltose-binding protein, thioridazine, and protein A, can also act as molecular chaperones to aid in ...

Creating an expression construct
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.3.2, pp. 76-77 [ doi:10.1107/97809553602060000810 ]
... the gene of interest. This is not nearly as daunting a task as it once was; an intense effort is now ... at genome sequencing and the preparation of cDNA clones from a number of prokaryotic and eukaryotic organisms. There are also a large number of cloned viral genes and genomes. This ...

Choosing an expression system
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.3.1, p. 76 [ doi:10.1107/97809553602060000810 ]
... in E. coli, yeast and insect cells (baculoviruses) differ to a greater or lesser extent from those used in higher eukaryotes ... organisms. Quite often the protein chosen for investigation comes from a higher eukaryote or from a virus that replicates in higher eukaryotes. The experimentalist prefers ...

Engineering an expression construct
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.3, pp. 76-77 [ doi:10.1107/97809553602060000810 ]
... in E. coli, yeast and insect cells (baculoviruses) differ to a greater or lesser extent from those used in higher eukaryotes ... organisms. Quite often the protein chosen for investigation comes from a higher eukaryote or from a virus that replicates in higher eukaryotes. The experimentalist prefers ...

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