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 Results for DC.creator="A." AND DC.creator="M." AND DC.creator="Stock" in section 3.1.4 of volume F
Mammalian cells
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.4.4, pp. 84-85 [ doi:10.1107/97809553602060000810 ]
... prepared by transfection; following transfection, some of the cells (usually a small percentage) will incorporate transfected DNA into their genomes. A number of agents can be used to transfect DNA; these ... limited to, CaPO4, DEAE Dextran, cationic lipids etc. This is a complex and poorly defined process; the transfected DNA is ...

Baculoviruses and insect cells
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.4.3, pp. 83-84 [ doi:10.1107/97809553602060000810 ]
... E. coli do not apply: there is no need for a Shine-Dalgarno sequence, no major problems with codon usage and fewer problems with a lack of appropriate chaperones. Although glycosylation is not the same ... monographs: Baculovirus Expression Protocols (Richardson, 1995) and Baculovirus Expression Vectors: A Laboratory Manual (O'Reilly et al., 1992). There ...

Yeast
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.4.2, pp. 82-83 [ doi:10.1107/97809553602060000810 ]
... the culture medium. However, degradation of secreted proteins can be a major problem. In some instances, proteolysis has been minimized by ... eukaryotes. In yeast, as in E. coli, cDNA sequences from a higher eukaryote must be tailored for high-level expression, following ... well understood. Yeast grows at 25-30C and has a slower growth rate than E. coli (under typical growth ...

E. coli
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.4.1, pp. 77-82 [ doi:10.1107/97809553602060000810 ]
... issues related to design of the plasmid, then continue with a discussion of fermentation conditions, and finally address some of the problems commonly encountered and potential solutions. Basically, a plasmid is a small circular piece of DNA. To be retained by ...

Expression systems
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.4, pp. 77-85 [ doi:10.1107/97809553602060000810 ]
... issues related to design of the plasmid, then continue with a discussion of fermentation conditions, and finally address some of the problems commonly encountered and potential solutions. Basically, a plasmid is a small circular piece of DNA. To be retained by ...

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