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 Results for DC.creator="A." AND DC.creator="M." AND DC.creator="Stock" in section 3.1.5 of volume F
Purifying and refolding denatured proteins
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.5.3, pp. 87-88 [ doi:10.1107/97809553602060000810 ]
... not with the purification of the protein, but in finding a proper way to refold it. Various general procedures for refolding ... specific protocols. The insoluble inclusion bodies are usually solubilized in a powerful chaotropic agent like guanidine hydrochloride or urea. In general ... in the buffer to accelerate correct pairing of disulfides. After a refolding procedure, the properly folded soluble protein must be ...

Affinity purification
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.5.2, p. 87 [ doi:10.1107/97809553602060000810 ]
... be possible to chemically link the substrate or product of a particular enzyme to an inert support. If the modification to ... the same substrate used to prepare the column. This is a powerful procedure and can produce greater than 100-fold purification in a single step. Although this is a fairly well developed ...

Conventional protein purification
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.5.1, pp. 85-87 [ doi:10.1107/97809553602060000810 ]
... natural sources, using conventional (as opposed to affinity) chromatography, where a 5000-fold purification was not unusual and the purifications routinely ... have improved both the speed and ease of protein purification. A wide variety of chromatography media (and prepacked columns) are commercially ... sample detection, fraction collection and information storage and output into a single integrated system, but such systems are relatively expensive. ...

Protein purification
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.5, pp. 85-88 [ doi:10.1107/97809553602060000810 ]
... natural sources, using conventional (as opposed to affinity) chromatography, where a 5000-fold purification was not unusual and the purifications routinely ... have improved both the speed and ease of protein purification. A wide variety of chromatography media (and prepacked columns) are commercially ... sample detection, fraction collection and information storage and output into a single integrated system, but such systems are relatively expensive. ...

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