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 Results for DC.creator="A." AND DC.creator="McPherson" in section 4.1.3 of volume F
Miniaturization, automation and robotics
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.3.6, p. 107 [ doi:10.1107/97809553602060000812 ]
... in most cases, nanolitres in some. This, in turn, produces a requirement for automated, microphotographic visualization instruments, and complex storage and ... handling systems, and their associated expense. On the other hand, a great advantage emerges in that they can perform enormous numbers ... little biological sample. This, in turn, relieves the investigator of a significant burden in terms of preparing and purifying macromolecules. ...

Free-interface and counter-diffusion methods
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.3.5, p. 107 [ doi:10.1107/97809553602060000812 ]
... Fig. 4.1.3.1d,e). To avoid too rapid mixing in a free-interface diffusion assay, the less dense solution is poured ... solution in contact will then diffuse into the other compartment. A more versatile version of this technique is counter-diffusion crystallization ... in its first version (Garcia-Ruiz & Moreno, 1994). In a typical experiment, a gel base is formed from agarose ...

Vapour-diffusion methods
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.3.4, p. 106 [ doi:10.1107/97809553602060000812 ]
... the favoured method of many experimenters. It is practised in a variety of forms and is the method of choice for robotic applications. In all of its versions, a drop with the protein to be crystallized, together with buffer, crystallant and additives, is equilibrated against a reservoir containing a solution of crystallant at a higher ...

Crystallization arrangements and classical methodologies
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.3, pp. 104-107 [ doi:10.1107/97809553602060000812 ]
... methods can be used to crystallize macromolecules (Ducruix & Giegé, 1999; McPherson, 1982, 1999). They all aim to bring the macromolecules ... 4.1.3.1 | | Principles of major methods used to crystallize biological macromolecules. (a) Two versions of batch crystallization. (b) Dialysis method with Cambridge ... Counter-diffusion setup in X-ray capillary tubes pinched in a gel layer. (e) Crystallization by free-interface diffusion in ...

Batch crystallizations
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.3.2, p. 105 [ doi:10.1107/97809553602060000812 ]
... geometries and samples from the ml to the µl range (McPherson, 1982; Luft et al., 1999a). Because one necessarily begins ... between the mother liquor and any solid surfaces results in a lower number of nucleation sites and larger crystals. Note that a hands-free system based on microbatch crystallization in modified ...

Historical development of methods
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.3.1, pp. 104-105 [ doi:10.1107/97809553602060000812 ]
... an old field that started more than 100 years ago (McPherson, 1991). Early methods included protein extractions with salts or ... Batch crystallization was the method of choice at that time. A first breakthrough that paralleled the development of X-ray methods ... crystallization experiments were developed (Carter & Carter, 1979; Carter, 1997). A second series of breakthroughs, in the mid-1980s, was ...

Dialysis methods
Sauter, C., Lorber, B., McPherson, A. and Giegé, R.  International Tables for Crystallography (2012). Vol. F, Section 4.1.3.3, p. 105 [ doi:10.1107/97809553602060000812 ]
... follow the same general principle. The protein is separated from a large volume of solvent by a semi-permeable membrane that allows passage of small molecules, but ... of the dialysis cell. The simplest technique is to use a dialysis bag (e.g. of inner diameter 2mm), but this ...

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