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 Results for DC.creator="S." AND DC.creator="H." AND DC.creator="Hughes" in section 3.1.3 of volume F
Addition of tags or domains
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.3.3, p. 77 [ doi:10.1107/97809553602060000810 ]
... CBP), cellulose-binding domain (CBD), chitin-binding domain (CBD), glutathione S-transferase (GST), maltose-binding protein (MBP), protein A domains, ribonuclease A S-peptide (S-tag) and thioridazine (Trx)] have already been engineered into ...

Creating an expression construct
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.3.2, pp. 76-77 [ doi:10.1107/97809553602060000810 ]
... RNA intermediate, thus removing unwanted introns (Shimotohno & Temin, 1982; Sorge & Hughes, 1982). If a good sequence is not available, one ... cassette mutagenesis using Bsp MI or a related enzyme; Boyer & Hughes, 1996). Ordinarily, however, these alternative strategies are only useful ... PCR after they have been cloned. References Boyer, P. L. & Hughes, S. H. (1996). Site-directed mutagenic analysis of ...

Choosing an expression system
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.3.1, p. 76 [ doi:10.1107/97809553602060000810 ]
Choosing an expression system 3.1.3.1. Choosing an expression system The first step in developing an expression strategy is the choice of an appropriate expression system, and this decision is critical. As we will discuss briefly below, the rules and/or sequences necessary to express RNA and proteins in E. coli, yeast ...

Engineering an expression construct
Hughes, S. H. and Stock, A. M.  International Tables for Crystallography (2012). Vol. F, Section 3.1.3, pp. 76-77 [ doi:10.1107/97809553602060000810 ]
... RNA intermediate, thus removing unwanted introns (Shimotohno & Temin, 1982; Sorge & Hughes, 1982). If a good sequence is not available, one ... cassette mutagenesis using Bsp MI or a related enzyme; Boyer & Hughes, 1996). Ordinarily, however, these alternative strategies are only useful ... CBP), cellulose-binding domain (CBD), chitin-binding domain (CBD), glutathione S-transferase (GST), maltose-binding protein (MBP), protein A domains, ...

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