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Introduction
van Heel, M., Portugal, R., Rohou, A., Linnemayr, C., Bebeacua, C., Schmidt, R., Grant, T. and Schatz, M.  International Tables for Crystallography (2012). Vol. F, Section 19.9.1, p. 624 [ doi:10.1107/97809553602060000875 ]
... Introduction During the 1950s and 1960s the electron microscope became a routine research instrument. Many biological structures we now take for ... Jacques Dubochet and co-workers (Adrian et al., 1984) represented a huge improvement in structural preservation for biological specimens in the ... in full action, in different conformational states, and may represent a window into the detailed functioning of the complexes in ...

Discussion
van Heel, M., Portugal, R., Rohou, A., Linnemayr, C., Bebeacua, C., Schmidt, R., Grant, T. and Schatz, M.  International Tables for Crystallography (2012). Vol. F, Section 19.9.11, p. 627 [ doi:10.1107/97809553602060000875 ]
... . The processing of 4D data sets, however, requires solving a large set of 3D structures simultaneously and that is necessarily a complex matter. The researcher will have to understand the basic ... and its pitfalls, and will thus have to go through a steep learning curve. The basic methodology (and the use ...

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