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International
Tables for Crystallography Volume F Crystallography of biological macromolecules Edited by M. G. Rossmann and E. Arnold © International Union of Crystallography 2006 |
International Tables for Crystallography (2006). Vol. F. ch. 19.4, p. 443
Section 19.4.5.3. Solvent conditions
aDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA, and bDepartments of Chemistry and Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520, USA |
Background arises from the incoherent scattering of hydrogen. Consequently, the use of high levels of D2O in the buffer improves the background, but may not be innocuous, since high levels of D2O have been seen to induce aggregation. In cases where complexes with labelled subunits are measured, it is advantageous to suppress small-angle background scatter by contrast matching the unlabelled regions via solvent adjustment.
