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INTERNATIONAL TABLES for CRYSTALLOGRAPHY |
Volume G |
Definition and Exchange of Crystallographic Data |
Description:
Data items in the EM_VITRIFICATION category record details about the method and cryogen used in rapid freezing of the sample on the grid prior to its insertion in the electron microscopeExample:
Example 1 - based on PDB entry 1DYL and laboratory records for the
structure corresponding to PDB entry 1DYL
_em_vitrification.entry_id 1DYL
_em_vitrification.id 1
_em_vitrification.sample_preparation_id 1
_em_vitrification.cryogen_name "ETHANE"
_em_vitrification.humidity 90
_em_vitrification.temp 95
_em_vitrification.instrument .
_em_vitrification.method "PLUNGE VITRIFICATION"
_em_vitrification.time_resolved_state .
_em_vitrification.citation_id 1
_em_vitrification.details
; SAMPLES WERE PREPARED AS THIN
LAYERS OF VITREOUS ICE AND
MAINTAINED AT NEAR LIQUID NITROGEN
TEMPERATURE IN THE ELECTRON MICROSCOPE
WITH A GATAN 626-0300 CRYOTRANSFER
HOLDER.
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Mandatory category: no
Copyright © 2005 International Union of Crystallography